Description: CD8⁺ T cell accumulation in tumors is essential for effective immunotherapy. However, the mechanisms of lymphocyte transit are still unclear. This study examined lymphatic vessel-mediated effector CD8⁺ T cell exit limits the accumulation of a broad repertoire of functional CD8⁺ T cells that improve tumor control in combination with immune checkpoint blockade. They showed that tumor-associated lymphatic vessels direct CD8⁺ T cell sequestration at the tumor periphery. This dataset includes flow cytometry, TCR sequencing, RNA sequencing, and immunohistochemistry data. The data indicate that the lymphatic vasculature helps tune the diversity and functional state of the intratumoral CD8⁺ T cell repertoire.

Subject of Study

Subject Domain

Keywords

Access

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Associated Publications

Steele MM, Dryg ID, Murugan D, Femel J, du Bois H, Hill C, Leachman SA, Chang YH, Coussens LM, Lund AW. T cell Egress via Lymphatic Vessels Limits the Intratumoral T cell Repertoire in Melanoma. bioRxiv [Preprint]. 2022 May 30:2022.05.30.494080.

Data Type

Biospecimens

Genomic

Imaging

Equipment Used

Agilent 2100 Bioanalyzer

Agilent 2200 TapeStation System

Applied Biosystems StepOne

BD FACSAria Fusion

BD Influx Cell Sorter

BD LSR II

BD LSRFortessa

Bio-Rad CFX384 Touch

Illumina HiSeq 2500

Illumina NextSeq 500

Illumina NovaSeq 6000

Keyence BZ-X810

Leica Aperio ImageScope AT

MoFlo XDP Cell Sorter

Software Used

CellProfiler v3.5.1

ComplexHeatmap

Cufflinks v2.2.1

DESeq2

FACSDiva

fgsea

Fiji

GraphPad Prism v9.0

HTSeq v0.11.2

MiXCR

msigdbr

pheatmap

Seurat v4

STAR v2.6.0a

STAR v2.7.3a

tcR

UMAP

Grant Support: P30 CA016087/NCI NIH

P30 CA069533/NCI NIH

R01 CA238163/NCI NIH

T32 CA106195/NCI NIH

T32 GM136542/NIGMS NIH

Lloyd J. Old STAR Program/Cancer Research Institute

403181/Melanoma Research Alliance

Brenden-Colson Center for Pancreatic Care/Brenden-Colson Center for Pancreatic Care

SU2C-AACR-DT14-14/American Association for Cancer Research

2016-00215/Swedish Research Council