NYU Dataset

Lymphatic Vessels Limits the Intratumoral T cell Repertoire in Melanoma

Part of: Lund Lab |
UID: 10601
* Corresponding Author
Description

CD8+ T cell accumulation in tumors is essential for effective immunotherapy. However, the mechanisms of lymphocyte transit are still unclear. This study examined lymphatic vessel-mediated effector CD8+ T cell exit limits the accumulation of a broad repertoire of functional CD8+ T cells that improve tumor control in combination with immune checkpoint blockade. They showed that tumor-associated lymphatic vessels direct CD8+ T cell sequestration at the tumor periphery. This dataset includes flow cytometry, TCR sequencing, RNA sequencing, and immunohistochemistry data. The data indicate that the lymphatic vasculature helps tune the diversity and functional state of the intratumoral CD8+ T cell repertoire.

Subject of Study
Subject Domain
Keywords

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Associated Publications
Data Type
Equipment Used
Agilent 2100 Bioanalyzer
Agilent 2200 TapeStation System
Applied Biosystems StepOne
BD FACSAria Fusion
BD Influx Cell Sorter
BD LSR II
BD LSRFortessa
Bio-Rad CFX384 Touch
Illumina HiSeq 2500
Illumina NextSeq 500
Illumina NovaSeq 6000
Keyence BZ-X810
Leica Aperio ImageScope AT
MoFlo XDP Cell Sorter
Software Used
CellProfiler v3.5.1
ComplexHeatmap
Cufflinks v2.2.1
DESeq2
FACSDiva
fgsea
Fiji
GraphPad Prism v9.0
HTSeq v0.11.2
MiXCR
msigdbr
pheatmap
R
Seurat v4
STAR v2.6.0a
STAR v2.7.3a
tcR
UMAP
Grant Support
Lloyd J. Old STAR Program/Cancer Research Institute
403181/Melanoma Research Alliance
Brenden-Colson Center for Pancreatic Care/Brenden-Colson Center for Pancreatic Care
SU2C-AACR-DT14-14/American Association for Cancer Research
2016-00215/Swedish Research Council