This study described karyotype CRISPR engineered aneuploidy technology (KaryoCreate), a system that enables generation of chromosome-specific aneuploidies by co-expression of an sgRNA targeting chromosome-specific CENPA-binding ɑ-satellite repeats together with dCas9 fused to mutant KNL1. They designed unique and highly specific sgRNAs for 19 of the 24 chromosomes. To examine the activity of the predicted...

This study demonstrated that loss of LZTR1, or leukemia-associated mutants in the LZTR1 substrate and RAS GTPase RIT1 that escape degradation, drives hematopoietic stem cell expansion and leukemia in vivo. For the study, genome-scale CRISPR/Cas9 screen performed in different variants of the TF-1 cell line genetically-modified to express mutant forms of RIT1 (M90I or F82C) or depleted in LZTR1 by CRISPR/Cas9,...

The RAS family of small guanosine triphosphatases (GTPases) control a diverse network of signaling pathways essential for human development and adult tissue homeostasis. RAS-related GTPase RIT1 proteins promote mitogen-activated protein kinase (MAPK) hyperactivation, but this mechanism remains poorly understood. This study examined whether MEK1/2 inhibition attenuates MAPK signaling in RIT1 M90I mice...

This study performed a proteogenomic analysis of DNA, RNA and protein levels across primary tumor samples and cancer cell lines from different tumor types, a panel of isogenic non-tumorigenic human colon epithelial cells and normal tissues. They found tissue specificity in the RNA-level and protein-level compensation of genes are affected by somatic copy number alterations (SCNAs). Using the DNA–RNA...

The National Health Interview Survey is a cross-sectional household interview survey. Sampling and interviewing are continuous throughout each year. The sampling plan follows a multistage area probability design that permits the representative sampling of households and noninstitutional group quarters (e.g., college dormitories). NHIS addresses topics such as health status, use of health services,...

The Women's Health Initiative (WHI) is a longitudinal national health study sponsored by the National Heart, Lung, and Blood Institute (NHLBI) that focuses on strategies for preventing heart disease, breast cancer, colorectal cancer, and osteoporotic fractures in postmenopausal women in the United States. The study includes data from 161,808 women aged 50-79 years old at the time of enrollment. Available...

The Nurses' Health Studies (NHS) are long-running, questionnaire-based studies on women's health. The study was initiated in 1976 with the NHS cohort. The initial studies focus on cancer, cardiovascular disease and risk factors for chronic health issues in women. The latest cohort, NH3, has been expanded to include both male and female registered nurses and nursing students. Survey and biospecimen...

The Surveillance, Epidemiology, and End Results (SEER) Program at the National Cancer Institute (NCI) is the authoritative source of information on cancer incidence and survival in the United States. SEER collects data on cancer incidence and population data associated by age, sex, race, year of diagnosis, and geographic area from registries across the country, covering approximately 48% of the national...

This dataset includes structured, in-depth interviews with 24 physicians from diverse clinical backgrounds and geographic areas within the United States. These physicians were identified with purposive sampling strategies. Physicians who reported currently caring for patients through active surveillance were invited to participate. An interview guide was developed based on a literature review and previous...

The Clinical Proteomic Tumor Analysis Consortium (CPTAC) analyzes cancer biospecimens by mass spectrometry, characterizing and quantifying their constituent proteins, or proteome. Proteomic analysis for each CPTAC study is carried out independently by Proteomic Characterization Centers (PCCs) using a variety of protein fractionation techniques, instrumentation, and workflows. Mass spectrometry and...