This dataset was collected to show that direct RNA sequencing using nanopore arrays is an alternative to conventional RNA sequencing approaches, which are complicated by high gene density, overlapping reading frames, and complex splicing patterns. In direct RNA sequencing, individual polyadenylated RNAs are sequenced directly. For this study, direct RNA sequencing was used to profile the herpes simplex...

Adipose tissue macrophages (ATMs) change during metabolic stress and are thought to contribute to metabolic syndrome. This study examined ATM subpopulations in steady state and in response to nutritional and infectious challenges. They characterized the epididymal white adipose tissue (eWAT) lymphoid and myeloid cell populations under steady state using RNA sequencing to analyze the transcriptome of...

Cerebral cavernous malformation (CCM) lesions are caused by loss of function of CCM genes. Previous study showed that NOGOB receptor (NGBR) knockout in endothelial cells results in cerebrovascular lesions in the mouse embryo. However, the molecular mechanism by which NGBR regulates CCM1/2 expression has not been explained. This study used RNA sequencing analysis to determine the alteration of transcriptomic...

Caenorhabditis elegans (C. elegans) uses specialized mechanosensory neurons to detect food. These neurons release dopamine to suppress foraging and promote dwelling. This study identified genes highly expressed in dopaminergic food-sensing neurons and screened for defects in the food-triggered slowing behavior mediated by these neurons. They found a K2P-family potassium channel gene TWK-2 that mutates...

T follicular helper (TFH) cells are CD4+ T helper cells that are important for immune responses to infection and vaccination. This study examined the translational regulation required for CD4+ T helper cells to differentiate to TFH cells and its contribution to TFH cell participation in pathogenesis in autoimmune disease in animal models. The experiments for non-autoimmune studies were performed with...

Single‐cell RNA sequencing (scRNA‐seq) has not been effectively used for exploring the interactions between the host cell and viral pathogens in latently infected cells. Therefore, this study used scRNA‐seq and single‐molecule sensitivity fluorescent in situ hybridization technologies to investigate host single‐cell transcriptome changes upon the reactivation of a human neurotropic virus, herpes simplex...

The development of dendritic cells (DCs) are controlled by the growth factor Flt3 ligand (Flt3L) and its receptor Flt3. This study contained in vitro CRISPR/Cas9-based dropout genetic screening of DC differentiation from HoxB8-FL cells, in vitro validation of the inhibition of mammalian target of rapamycin (mTOR) signaling as a requirement for HoxB8-FL differentiation, and in vivo validation of select...

The cornea is the outermost transparent layer of the eye that serves as a protective barrier and studying the cornea can be crucial for understanding human corneal diseases. Although several studies have examined the single-cell transcriptomic composition of the human cornea, its organoids have not been examined similarly. This study explained the transcriptomic cell fate of human cornea organoids...

Previous studies in murine cardiomyocytes and ex vivo knockdown in zebrafish embryonic hearts have demonstrated that NOS1AP expression levels can alter cellular electrophysiology. Therefore, this study generated constitutive and heart muscle-restricted Nos1ap knockout mice to explore the role of NOS1AP in cardiac electrophysiology and QT interval regulation in vivo. To assess the impact of Nos1ap loss...