NYU Dataset
Direct RNA Sequencing Characterized Complex Viral Transcriptomes
Part of: Wilson Lab |
UID: 10474
- Description
This dataset was collected to show that direct RNA sequencing using nanopore arrays is an alternative to conventional RNA sequencing approaches, which are complicated by high gene density, overlapping reading frames, and complex splicing patterns. In direct RNA sequencing, individual polyadenylated RNAs are sequenced directly. Therefore, direct RNA sequencing was used to profile the herpes simplex virus 1 (HSV-1) transcriptome during productive infection of primary cells. This publication also contains supplementary data, which includes data on sequencing metrics, proximal transcription start sites (pTSS) for canonical HSV-1 open reading frames (ORFs), ORFs with multiple pTSS, novel pTSS, fusion transcripts, and primers used in the study.
Fibroblasts
ARPE-19 cells
hESC-derived neurons
GFP-Us11
Kos
17syn+
F
KOS N12 (ΔICP4)
Associated Publications
Data Type
Equipment Used
Software Used
Access
- Restrictions
-
Free to All
- Instructions
- Raw fast5, basecalled fastq (both nanopores), and Illumina fastq datasets generated as part of this study can be downloaded from the European Nucleotide Archive (ENA). All other data supporting the findings of this study are available within the article on PubMed Central (PMC) under Supplementary Materials.
- Grant Support
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JP17H05816/MEXT KAKENHIJP16H06429/MEXT KAKENHIJP16K21723/MEXT KAKENHIJP17K008858/JSPS KAKENHITakeda Science Foundation/Takeda Science FoundationDaiichi Sankyo Foundation/Daiichi Sankyo Foundation
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