Genome-Wide Screening Identifies Trim33 as an Essential Regulator of Dendritic Cell Differentiation
- Description
The development of dendritic cells (DCs) are controlled by the growth factor Flt3 ligand (Flt3L) and its receptor Flt3. This study contained in vitro CRISPR/Cas9-based dropout genetic screening of DC differentiation from HoxB8-FL cells, in vitro validation of the inhibition of mammalian target of rapamycin (mTOR) signaling as a requirement for HoxB8-FL differentiation, and in vivo validation of select screen hits by conditional gene targeting. All validation experiments were performed in biological replicates and animals were assigned to groups based on genotype. The dataset contains RNA sequencing, ATAC sequencing, and single guide RNA sequencing data. These data indicate that transcriptional regulator Trim33 controls Flt3L-driven dendritic cell differentiation.
Access
- Restrictions
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Free to All
- Instructions
- RNA sequencing, ATAC sequencing, and single guide RNA sequencing data have been deposited in Gene Expression Omnibus (GEO). All other data needed to support the conclusions of the paper are present on PubMed Central (PMC) under Supplementary Materials.
- Grant Support
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Bernard Levine Postdoctoral Fellowship in Immunology/NYU Grossman School of MedicineDamon Runyon Postdoctoral Research Fellowship/Damon Runyon Cancer Research Foundation