NYU Dataset

Cardiac Muscle–Restricted Partial Loss of Nos1ap Expression Has Limited Impact on Electrocardiographic Features

UID: 10714
* Corresponding Author
Description

Previous studies in murine cardiomyocytes and ex vivo knockdown in zebrafish embryonic hearts have demonstrated that NOS1AP expression levels can alter cellular electrophysiology. Therefore, this study generated constitutive and heart muscle-restricted Nos1ap knockout mice to explore the role of NOS1AP in cardiac electrophysiology and QT interval regulation in vivo. To assess the impact of Nos1ap loss on heart transcriptome during development, RNA sequencing was performed in heart tissue from wildtype (males; n=5) and Nos1ap null homozygous (males; n=5) embryonic day 13.5 (e13.5) mice, and assessed for differential gene expression. The data indicate that constitutive loss of Nos1ap has no major widespread impact on transcriptome in e13.5 hearts.

Subject of Study
Subject Domain
Keywords

Access

Restrictions
Free to All
Instructions
RNA sequencing data are accessible on Gene Expression Omnibus (GEO). All other data are provided in the paper on PubMed Central (PMC) under Supplementary Materials.
Access via GEO

RNA-seq data
Accession #: GSE210266

Access via PMC

Other data
Accession #: PMC10627271

Associated Publications
Data Type
Equipment Used
ADInstruments PowerLab 4/35
Applied Biosystems 7900HT
Applied Biosystems QuantStudio 5
FUJIFILM Vevo 2100 Imaging System
Illumina HiSeq 2500
Rodent Surgical Monitor+
Software Used
DESeq v1.24.0
ECG Analysis
FastQC v0.11.5
GENCODE
ImageJ
LabChart v8
QuantStudio Design and Analysis v1.2
RSEM v1.2.31
Sequence Detection System Software v2.1
STAR
Trimmomatic v0.36
Grant Support
McGovern Medical School at UTHealth/McGovern Medical School at UTHealth