NYU Dataset

Single-Cell Transcriptomics Identifies Gadd45b as a Regulator of Herpesvirus-Reactivating Neurons

UID: 10674
* Corresponding Author
Description

Single‐cell RNA sequencing (scRNA‐seq) has not been effectively used for exploring the interactions between the host cell and viral pathogens in latently infected cells. Therefore, this study used scRNA‐seq and single‐molecule sensitivity fluorescent in situ hybridization technologies to investigate host single‐cell transcriptome changes upon the reactivation of a human neurotropic virus, herpes simplex virus‐1 (HSV‐1). The dataset includes scRNA-seq of HSV-1 latency data. These data indicate HSV‐1 reactivation state in primary neurons is determined by a unique subcellular localization signature of the stress sensor Gadd45b.

Subject of Study
Subject Domain
Keywords

Access

Restrictions
Free to All
Instructions
All sequencing data generated as part of this study are available on European Nucleotide Archive (ENA).
Access via ENA

Sequencing data
Accession #: PRJEB39022

Associated Publications
Data Type
Equipment Used
Applied Biosystems StepOnePlus
Illumina HiSeq 4000
Illumina NovaSeq 6000
Nikon Eclipse Ti
Zeiss LSM 700
Software Used
Cell Ranger v3.0.0
Fiji
GraphPad Prism v8.0
ImageJ
MAST
R Markdown
R v4.0.0
RStudio
Seurat v3.0
Grant Support
687306/Melanoma Research Alliance
Simons Fellows Program/Simons Foundation