Description

Adipose tissue macrophages (ATMs) change during metabolic stress and are thought to contribute to metabolic syndrome. This study examined ATM subpopulations in steady state and in response to nutritional and infectious challenges. They characterized the epididymal white adipose tissue (eWAT) lymphoid and myeloid cell populations under steady state using RNA sequencing to analyze the transcriptome of purified eWAT monocytes, macrophages, and dendritic cells. This dataset includes RNA sequencing data. The data indicate that ATM populations dynamically adapt to metabolic stress and inflammation, which suggests that there is an important role for these cells in maintaining tissue homeostasis.

Subject of Study
Subject Domain
Keywords

Access

Restrictions
Free to All
Instructions
RNA sequencing data are deposited at the Gene Expression Omnibus (GEO) repository.
Access via GEO


Accession #: GSE126407

Associated Publications
Data Type
Equipment Used
BD FACSAria II
BD LSR II
Illumina HiSeq 2500
LaVision BioTec UltraMicroscope II
Olympus FV1000 MPE
OneTouch Glucose Meter
Zeiss Axio Observer
Zeiss LSM 710
Zeiss LSM 880
Software Used
Cytobank
edgeR
featureCounts
Fiji v1.0
FlowJo
goseq
GraphPad Prism
ImageJ
Imaris v8.0.1
Inspector Pro
limma
R
STAR v2.5.3a
Trimmomatic
ZEN
Grant Support
CNPq Fellowship/National Council for Scientific and Technological Development
CAPES Postdoctoral Fellowship/Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior