NYU Dataset

Translational Regulation of TFH Cell Differentiation and Autoimmune Pathogenesis

Part of: Schneider Lab |
UID: 10643
* Corresponding Author
Description

T follicular helper (TFH) cells are CD4+ T helper cells that are important for immune responses to infection and vaccination. This study examined the translational regulation required for CD4+ T helper cells to differentiate to TFH cells and its contribution to TFH cell participation in pathogenesis in autoimmune disease in animal models. The experiments for non-autoimmune studies were performed with 5 to 10 mice per group, while the experiments for autoimmune studies were performed with 10 to 20 mice per group. This dataset contains RNA sequencing data as well as supplementary data tied to the publication. The supplementary data includes raw and analyzed RNA sequencing data, list of mRNAs, viability dye, antibody clones, and dilutions.

Subject of Study
Subject Domain
Keywords

Access

Restrictions
Free to All
Instructions
Raw RNA sequencing data developed in this study are available from Gene Expression Omnibus (GEO). All other data are present in the paper on PubMed Central (PMC) under Supplementary Materials.
Access via GEO

Raw RNA-seq data
Accession #: GSE193652

Access via PMC

Other data
Accession #: PMC9232117

Associated Publications
Data Type
Equipment Used
Agilent 2100 Bioanalyzer
Akoya Biosciences Vectra Polaris
Bio-Rad ZE5
Illumina HiSeq 4000
Leica BOND RX
Thermo Scientific EVOS FL
Zeiss LSM 700
Software Used
DAVID
Enrichr
Fiji
FlowJo
GraphPad Prism
HTSeq v0.10.0
Metascape
PASTA
Phenochart
QuPath
RIVET
SAMtools v1.9
STAR v2.6.0a
Trimmomatic v0.36
Venny
Grant Support
Jan Vilcek/David Goldfarb Fellowship/NYU Langone Health
Judith & Stewart Colton Center for Autoimmunity/NYU Langone Health
Bernard Levine Postdoctoral Fellowship in Immunology/NYU Grossman School of Medicine
LLS Scholar Award/Leukemia & Lymphoma Society