NR2F2 Isoform Unleashes a Metastatic Program in Melanoma
- Description
This study described DNA methylation changes that accompany metastatic progression in melanoma patients and found localized methylation changes in the regulatory region of NR2F2. NR2F2 is an orphan-nuclear receptor that is essential for embryonic and neural crest cell (NCC) development. For this study, they examined DNA methylation profiles of NCCs and melanocytes as well as melanoma patient tissues from primary and metastatic sites. This dataset contains methylation array, ChIP sequencing, and RNA sequencing data.
The publication also includes supplementary data, which contains two files. The first file includes genes consistently down‐regulated in shNR2F2‐Iso2 compared to shSCR and 12‐273BM melanoma cells. The second file includes location of NR2F2 ChIP sequencing peaks with NR2F2‐like motifs, prediction of NR2F2 target genes with GREAT, intersection of GREAT predicted NR2F2 target genes with mRNA up‐regulated or down‐regulated after shNR2F2‐iso knock‐down, DAVID‐Gene Ontology of GREAT predicted NR2F2 target genes that increased after NR2F2‐iso2 knock‐down, DAVID‐Gene Ontology of GREAT predicted NR2F2 target genes that decreased after NR2F2‐iso2 knock‐down, DAVID‐Gene Ontology of the 3000 most differentially expressed, and GREAT predicted NR2F2 target genes after NR2F2‐iso2 knock‐down.
Access
- Restrictions
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Free to All
- Instructions
- The methylation array and sequencing data, including ChIP sequencing and RNA sequencing data are deposited in Gene Expression Omnibus (GEO) database. All other data are available in the article under Supplementary Materials.
- Grant Support
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PIOF-GA-2013-623443/European Union