NYU Dataset

GADD34 Promotes Fast Dephosphorylation of eIF2α and Elevation of Neuronal Protein Synthesis

Part of: Klann Lab |
UID: 10697
* Corresponding Author
Description

This study examined the molecular pathways of translational control that are triggered by neuronal stimulation with brain-derived neurotrophic factor (BDNF). For the study, primary rodent neurons were exposed to BDNF and then examined for GADD34 protein levels. GADD34 is a protein phosphatase 1-targeting protein that directly binds to eukaryotic initiation factor 2α (eIF2α). This promotes eIF2α dephosphorylation and increases in de novo protein synthesis that supports memory consolidation. The dataset includes TRAP and RNA sequencing data. The results reveal that increases in protein synthesis resulting from neuronal activation require GADD34-induced eIF2α dephosphorylation.

Subject of Study
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Access

Restrictions
Free to All
Instructions
Access to original TRAP-seq and RNA-seq data used in the paper are freely available on Gene Expression Omnibus (GEO).
Access via GEO

TRAP-seq and RNA-seq data
Accession #: GSE248013

Associated Publications
Data Type
Equipment Used
Agilent TapeStation System
Bio-Rad CFX96 Touch
Bio-Rad Model EM-1 Econo UV Monitor
Biocomp Piston Gradient Fractionator
Gilson FC 203B Fraction Collector
Illumina NovaSeq 6000
Leica SP8 LIGHTNING
Software Used
clusterProfiler
ComBat-seq
Cutadapt v1.8.2
DESeq2
GraphPad Prism v9.3.1
ImageJ
pheatmap
STAR v2.7.7a
SynGO
Grant Support