NYU Dataset

Spatial Transcriptomics Stratifies Psoriatic Disease Severity

Part of: GTC Pre-Publication Pilot | An internal pilot project to encourage sharing of pre-publication data generated by the Genome Technology Center (GTC)
UID: 10587
* Corresponding Author
Description

This study described human psoriasis (PsO), which is a prototypic immune-mediated condition with a high predilection for extra-cutaneous involvement. Although the cellular and molecular features of human inflammatory skin diseases have been identified, their tissue context and systemic impact remains unclear. Spatial transcriptomics was performed to explain the cellular landscape of both healthy and psoriatic skin. They analyzed 25 healthy, active lesion, and clinically uninvolved skin biopsies for this study. This dataset includes single cell RNA sequencing data. The data suggests that mild and severe forms of PsO have distinct molecular features and severe PsO may profoundly alter the cellular and metabolic composition of distal unaffected skin sites.

Subject of Study
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Access

Restrictions
Free to All
Instructions
RNA sequencing data is publicly available at Gene Expression Omnibus (GEO). Code used for data analysis of single cell and spatial RNA sequencing, and RDS files and other processed data are deposited in Zenodo. All other data needed to support the conclusions in this paper can be found on PubMed Central (PMC) under Supplementary Materials.
Access via GEO

RNA-seq data
Accession #: GSE202011

Access via Zenodo

RDS files and other processed data

Access via PMC

Other data
Accession #: PMC10502701

Associated Publications
Data Type
Equipment Used
Illumina NovaSeq 6000
Leica SCN400F
Software Used
clusterProfiler
DESeq2
enrichR v3.0
Harmony
pheatmap
scran
Seurat
Space Ranger
xCell
Grant Support
Pew-Stewart Scholars for Cancer Research/Pew Charitable Trusts
Translational Immunology Center/NYU Langone Health
Robertson Stem Cell Investigator Award/New York Stem Cell Foundation
Packard Fellowship for Science and Engineering/David and Lucile Packard Foundation
Scientist Development Award/Rheumatology Research Foundation
Psoriatic Disease Research Fellowship/National Psoriasis Foundation
Early Career Research Grant/National Psoriasis Foundation
Pilot Research Grant in Rheumatology/Dermatology/Group for Research and Assessment of Psoriasis and Psoriatic Arthritis