MicroRNA-33 Silencing Reprograms the Immune Cell Landscape in Atherosclerotic Plaques
- Description
MicroRNA-33 (miR-33) targeted inhibition increases plasma HDL cholesterol and promotes atherosclerosis regression by enhancing reverse cholesterol transport and dampening plaque inflammation. This study showed how miR-33 inhibition alters the dynamic balance and transcriptional landscape of immune cells in atherosclerotic plaques. They used single cell RNA sequencing of aortic CD45+ cells, combined with immunohistologic, morphometric, and flow cytometric analyses to define the changes in plaque immune cell composition, gene expression, and function following miR-33 inhibition. This dataset includes RNA sequencing data. This study provides insight into the immune mechanisms and cellular players that execute anti-miR-33’s atheroprotective actions in the plaque.
Access
- Restrictions
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Free to All
- Instructions
- RNA sequencing data have been deposited in Gene Expression Omnibus (GEO).
- Grant Support
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Schl 2172/2/German Research FoundationNFL-2020-218415/Chan Zuckerberg Initiative19POST34380010/American Heart Association14POST20180018/American Heart Association19CDA34630066/American Heart Association20SFRN35210252/American Heart Association