Description

Previous study indicated that the receptor for advanced glycation end products (RAGE) in the progression and regression of atherosclerosis. Therefore, this study examined if Diaphanous 1 (DIAPH1) binds to the RAGE cytoplasmic domain and contributes to progression of atherosclerosis. They intercrossed atherosclerosis prone Ldlr−/− mice with mice devoid of Diaph1 and fed them Western diet for 16 weeks. The dataset includes flow cytometry, immunohistochemistry, and RNA sequencing data. The publication also contains supplementary data. The supplementary data includes source data, uncropped blots, and differentially expressed genes in Ldlr−/− Diaph1−/− mice versus Ldlr−/− mice.

Subject of Study
Subject Domain
Keywords

Access

Restrictions
Free to All
Instructions
Supporting data for the associated publication is available in PubMed Central (PMC) under Supplementary Materials. RNAseq data can be accessed from NCBI's Gene Expression Omnibus (GEO) data repository.
Access via GEO

RNAseq data
Accession #: GSE156403

Associated Publications
Data Type
Equipment Used
Applied Biosystems 7300
BD LSR II
Illumina HiSeq 2500
Keyence BZ-X800
Leica ASP300S
Leica BOND RX
Leica PELORIS
LI-COR Odyssey Imaging System
Lunar PIXImus DEXA
Molecular Devices SpectraMax
NanoZoomer Digital Slide Scanner
Shimadzu FPLC System
Ventana Discovery XT
Zeiss Axioplan
Software Used
Cluster v3.0
featureCounts
FlowJo v10.8.1
GO Enrichment Analysis
GraphPad Prism v8.2.0
Image Studio
Image-Pro Plus v7.0
ImageJ
iPathwayGuide
Java TreeView
Limma-Voom
OMERO Plus
Signaling Pathway Impact Analysis (SPIA)
WebGestalt
Dataset Format(s)
PDF, Microsoft Excel
Grant Support
Diabetes Research Program/NYU Langone Health