This study characterized gut microbiome in pre-treatment stool using 16S rRNA gene and shotgun metagenome sequencing in melanoma patients undergoing immunotherapy. Transcriptional expression of metagenomic pathways was confirmed with metatranscriptome sequencing. They also examined associations of taxa and metagenomic pathways with progression-free survival (PFS). This dataset contains 16S rRNA gene...

CD8+ T cell accumulation in tumors is essential for effective immunotherapy. However, the mechanisms of lymphocyte transit are still unclear. This study examined lymphatic vessel-mediated effector CD8+ T cell egress limits the accumulation of a broad repertoire of functional CD8+ T cells. To investigate the transcriptional changes in lymphatic endothelial cells (LECs) during tumor development, RNA...

Although targeted cancer therapies have been successful, their efficacies are limited by the development of resistance driven by clonal evolution within tumors. This study developed a single-cell barcoding approach to comprehensively trace clonal dynamics and capture live lineage-coupled resistant cells for in-depth multi-omics analysis and functional exploration. They showed that heterogeneous clones,...

Since 2002, the Interdisciplinary Melanoma Cooperative Group (IMCG) at Perlmutter Cancer Center has maintained one of the largest clinicopathologic resources, the Melanoma Clinicopathological-Biospecimen Database and Repository, for research on patients 18 years old and over with melanoma or at high risk for melanoma. Clinical data is stored in a secure REDCap database which contains 653 fields to...

This study described DNA methylation changes that accompany metastatic progression in melanoma patients and found localized methylation changes in the regulatory region of NR2F2. NR2F2 is an orphan-nuclear receptor that is essential for embryonic and neural crest cell (NCC) development. For this study, they examined DNA methylation profiles of NCCs and melanocytes as well as melanoma patient tissues...

Resident memory T cells (TRM) provide localized protection due to their position in barrier tissues. However, interstitial signals necessary for their formation and persistence are not properly understood. This study demonstrated that antigen-dependent induction of the chemokine receptor, CXCR6, is a conserved requirement for TRM formation in peripheral tissue after viral infection. Cutaneous murine...

In order to understand the immune-spatial environment of melanoma sentinel lymph nodes (SLN), this study profiled 43 tissues samples from a cohort Stage II melanoma that underwent SLN biopsy. All samples were randomly selected from archived samples between 2010-2017 at the University of Pennsylvania. The median age of the cohort was 61 and 76% were male. 22 lacked tumor infiltration (SLN-) 21 contained...