Unravelling Cysteine-Deficiency-Associated Rapid Weight Loss
- Description
The growing interest in diets that induce weight loss prompted this study to compare restriction of each individual essential amino acid (EAA) and cysteine. For the study, each of the essential amino acids (EAAs) and cysteine were individually removed from diet in Cse−/− and Cse+/− mice to induce weight loss. To evaluate the molecular responses triggered by cysteine deprivation, bulk RNA-sequencing analysis was performed on liver, muscle, and adipose tissue. After a 3-day calorie restriction (CR) control diet, Cse−/− and Cse+/− mice were shifted to either CR control, CR cysteine-free, or CR tryptophan-free diets, and tissues were collected after 2 days. To further characterize the weight loss, metabolic assessments were performed on Cse−/− and Cse+/− mice. After adapting to CR control diet, the mice were placed into metabolic cages and switched to the CR cysteine-free diet 2 days later. This dataset includes RNA sequencing and metabolomics data.
Access
- Instructions
- RNA sequencing data generated and used for this project are available at Gene Expression Omnibus (GEO) and metabolomics data generated and used for this project are available at Metabolomics Workbench. Source data are provided with this paper on PubMed Central (PMC).
- Grant Support
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Howard Hughes Medical Institute/Howard Hughes Medical InstituteBlavatnik Family Foundation/Blavatnik Family FoundationRGPIN-2023-05099/Natural Sciences and Engineering Research Council of CanadaCGCATF-2021/100022/Cancer Research UK/National Cancer InstituteRSG-21-115-01-MM/American Cancer Society