Human Hepatoma Huh-7 Cell Culture Models Deficient in Apolipoprotein B Secretion
- Description
Apolipoprotein B (apoB) is an essential structural protein for the assembly and secretion of triglyceride-rich lipoproteins. Inhibition of apoB and microsomal triglyceride transfer protein (MTP) decreases plasma lipid levels but at a risk of lipid accumulation in tissues. To understand the global consequences of APOB gene deficiency, a human hepatoma (Huh-7) cell culture model system deficient in apoB was generated. ApoB deficient Huh-7 cells (Ako-3 cells) were generated using the CRISPR/Cas9 system. Total RNA for transcriptomics and overnight conditioned media for proteomics was obtained from the Ako-3 and Huh-7 cells. In addition, total transcriptome analysis for the MTP deficient Huh-7 cells (Mko-3 cells) was performed, since both APOB and MTP are important part of the lipoprotein assembly and secretion.
Access
- Restrictions
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Free to All
- Instructions
- RNA sequencing data have been deposited in Gene Expression Omnibus (GEO).
- Grant Support