NYU Dataset

Genomic Analysis of Group B Streptococcus Carriage Isolates From Botswana

Part of: Ratner Lab |
UID: 10746
* Corresponding Author
Description

Group B Streptococcus (GBS) is a major cause of neonatal sepsis and meningitis and a leading cause of death in infants globally. Rectovaginal colonization in late pregnancy can lead to transmission to newborns and is the major risk factor for early-onset GBS disease. This study includes genomic analysis of GBS isolated from pregnant people in Botswana. GBS isolates were examined by whole genome sequencing to determine multilocus sequence typing/clonal complex (MLST/CC), presence of specific bacterial virulence determinants, and antimicrobial susceptibility. Sequence type [ST] 223 was determined to be the predominant ST, followed by ST24 and ST17. Most common CCs were CC23, CC17, and CC452. A minority of isolates had MLST profiles not previously described. These were deposited in the PubMLST database and assigned STs 2126 (strain AR1534), 2140 (strain AR1537), and 2145 (strain AR1563). The dataset contains raw sequencing reads, which highlight the importance of local epidemiologic studies of GBS.

Geographic Coverage
Botswana
Subject of Study
Subject Domain
Keywords

Access

Restrictions
Free to All
Instructions
Raw sequencing reads are available in the Sequence Read Archive (SRA) through BioProject.
Access via BioProject

Raw sequencing reads
Accession #: PRJNA986888

Associated Publications
Data Type
Equipment Used
Illumina NovaSeq 6000
Thermo Scientific KingFisher Flex
Software Used
Mashtree v1.2.0
SRST2 v0.2.0
Trimmomatic v0.36
Grant Support
Division of Neonatology/Carver College of Medicine
Melissa Ketunuti Memorial Global Health Fund/Children's Hospital of Philadelphia
PIDS Summer Research Scholars Award/Pediatric Infectious Diseases Society
Pediatrics Summer Programs for Medical Students/NYU Langone Health
Other Resources
PubMLST

Isolate data