NYU Dataset

Proteogenomic Analysis of Cancer Aneuploidy and Normal Tissues

Part of: Davoli Lab |
UID: 10722
* Corresponding Author
Description

This study performed a proteogenomic analysis of DNA, RNA and protein levels across primary tumor samples and cancer cell lines from different tumor types, a panel of isogenic non-tumorigenic human colon epithelial cells and normal tissues. They found tissue specificity in the RNA-level and protein-level compensation of genes are affected by somatic copy number alterations (SCNAs). Using the DNA–RNA and RNA–protein correlations, they inferred the degree of regulation at the RNA and protein levels. By revealing how cells regulate RNA and protein abundances of genes with SCNAs, they provided insights into the rules of gene regulation. This publication includes supplementary data for gene compensation analysis, DNA–RNA correlation and RNA–protein correlation analysis, complete list of cellular pathways and related analyses, and complete list of t-value and Gene Set Enrichment Analysis.

Subject of Study
Subject Domain
Keywords

Access

Restrictions
Free to All
Instructions
All study data are included in the article on PubMed Central (PMC) under Additional Files and codes used in this publication are available in GitHub.
Access via PMC

Study data
Accession #: PMC9491860

Associated Publications
Data Type
Equipment Used
Agilent 2100 Bioanalyzer
Agilent 2200 TapeStation System
Illumina NextSeq 500
Illumina NovaSeq 6000
LightCycler 480 System
Qubit 2.0 Fluorometer
Thermo Scientific Q Exactive HF-X
Software Used
boot v1.3-28
BWA v0.7.17
CopywriteR v1.18.0
GATK v4.1.7.0
GO Enrichment Analysis
GSEA v7.4
MaxQuant v1.6.15.0
MSFragger v3.0
msigdbr v7.4.1
MSstatsTMT
Perseus
Philosopher v3.2.8
ProteinProphet
R
RSEM v1.3.1
Spectrum Mill v7.0
STAR v2.7.7a
TMT-Integrator
Grant Support
Cancer Grand Challenges/Cancer Research UK
C5470/A27144/Mark Foundation for Cancer Research
322977937/GRK2344/German Research Foundation
818846 – ImmuNiche – ERC-2018-COG/European Research Council