NYU Dataset

RNA Sequencing Analysis of Caenorhabditis elegans DAF-18 During Primordial Germ Cell Quiescence

Part of: Jane Hubbard Lab |
UID: 10706
* Corresponding Author
Description

PTEN is one of the most frequently lost tumor suppressors in human cancers and regulates quiescence of stem cells and cancer cells. The Caenorhabditis elegans daf-18 gene encodes the worm version of PTEN. Previous studies showed that in worms that have lost daf-18 function, primordial germ cells (PGCs) inappropriately begin dividing in the L1 larvae hatched into starvation conditions. This study examined the role of daf-18 in maintaining PGC quiescence in L1 starvation. The dataset includes RNA sequencing and imaging data. The publication also includes supplementary data, which contains RNA sequencing results and analysis, and strains, plasmids and primers used in this study.

Subject of Study
Subject Domain
Keywords

Access

Restrictions
Free to All
Instructions
Raw and processed RNA sequencing data are available on Gene Expression Omnibus (GEO). All other data are provided in the paper on PubMed Central (PMC) under Supplementary Materials.
Access via GEO

Raw and processed RNA-seq data
Accession #: GSE151299

Access via PMC

Other data
Accession #: PMC8294487

Associated Publications
Data Type
Equipment Used
Illumina NovaSeq 6000
Zeiss Axio Imager Z1
Software Used
Bowtie v1.2.2
edgeR v3.24.3
GExplore v1.4
HTSeq v0.11.2
ImageJ
WormBase Enrichment Analysis
Grant Support
132083-PF-18-029-01-DDC/American Cancer Society
C029561/New York State Stem Cell Science