NYU Dataset

Ca2+ Channel ORAI1 Regulates Oral Cancer Growth and Nociceptive Pain

Part of: Lacruz Lab |
UID: 10664
* Corresponding Author
Description

This study described the function of the Ca2+ channel ORAI1 as an important regulator of oral cancer pain. Previous study showed that ORAI1 was highly expressed in tumor samples from patients with oral cancer. ORAI1 activation caused sustained Ca2+ influx in human oral cancer cells. To address the effects of ORAI1 in cell proliferation, HSC-3 cells were stimulated with 25 µM cyclopiazonic acid in the presence or absence of the ORAI inhibitor synta-66 for 3 hours before washing the cells and isolating the mRNA. The dataset includes RNA sequencing data, which indicates that ORAI1 regulated many genes encoding oral cancer markers such as metalloproteases and pain modulators.

Subject of Study
Subject Domain
Keywords

Access

Restrictions
Free to All
Instructions
The RNA sequencing data have been deposited in Gene Expression Omnibus (GEO).
Access via GEO

RNA-seq data
Accession #: GSE236706

Associated Publications
Data Type
Equipment Used
Bio-Rad CFX Connect
Bio-Rad T100 Thermal Cycler
Leica Aperio CS2
Leica SP8 LIGHTNING
Molecular Devices FlexStation 3
NanoEntek EVE
Nikon Eclipse Ti
Nikon Eclipse Ti2
Promega GloMax 9301
Thermo Scientific NanoDrop
WPI Plethysmometer
Software Used
DESeq2 v1.28.1
FastQC v0.11.5
GraphPad Prism v9.0
HTSeq v0.6.1p1
ImageJ
JMP Genomics
LAS X
NIS-Elements v5.20.01
STAR v2.5.2a
Trimmomatic v0.32
Grant Support
AD105/NYU Abu Dhabi