NYU Dataset

Targeting Succinate Receptor Effectively Inhibits Periodontal Disease

UID: 10605
* Corresponding Author
Description

Periodontal disease (PD) is induced by a bacterial dysbiosis in the oral cavity that stimulates inflammation and bone loss. This study showed elevation of succinate in PD, which aggravates the disease through the succinate receptor (SUCNR1). Metabolomics assay compared the succinate level in the subgingival plaque of patients with PD. RNAscope and immunofluorescence assays were conducted to validate the presence of SUCNR1 in both human and mouse gingival tissues. Loss and gain of function of SUCNR1 in PD were compared between SUCNR1 KO and WT mice. They developed a gel formulation of a small compound specifically blocking SUCNR1 to prevent and treat PD by inhibiting dysbiosis, inflammation, and bone loss. This dataset contains sequencing and immunohistochemistry data.

Subject of Study
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Keywords

Access

Restrictions
Free to All
Instructions
Raw sequence reads are deposited at Sequence Read Archive (SRA) through BioProject and microbiome data and code used for downstream analysis in the manuscript were deposited to Zenodo.
Access via BioProject

Raw sequence reads
Accession #: PRJNA772167

Access via Zenodo

Microbiome data and code
Accession #: 7019470

Associated Publications
Data Type
Equipment Used
Akoya Biosciences Vectra Polaris 3
Bio-Rad CFX384 Touch
Bruker Avance 400 MHz NMR
Bruker Avance 500 MHz NMR
Bruker Avance 600 MHz NMR
Leica BOND Polymer Refine Detection System
Leica BOND RX
Leica PELORIS
Leica RM2135
Leica RM2235
Liquid Chromatograph-Mass Spectrometer
MESO QuickPlex SQ 120
Molecular Devices SpectraMax M5
NanoZoomer 2.0-HT
Nikon Eclipse E600
SkyScan 1172
Thermo Scientific Dionex UltiMate 3000
Thermo Scientific EVOS
Thermo Scientific Q Exactive HF
Software Used
CTVox
Cytoscape v3.8.2
DataViewer
DISCOVERY WORKBENCH
ggplot2
GraphPad Prism v9.1.1
Greengenes v13.8
Hmisc v4.5.0
HOMD v15.2
ImageJ
inForm
MicrobiotaProcess v1.2.2
NRecon
OMERO
OMERO.figure
OMERO.insight
Phenochart
phyloseq v1.34
QIIME2 v2.0
R v4.0.5
vegan v2.5-7
Grant Support