Spike sequences are exemplified by memory replays in sharp-wave ripples (SWRs) and high-frequency oscillations resulting from pyramidal cell-interneuron interactions in the hippocampus. However, the microcircuitry mechanism that enables such flexible spike sequencing remains unknown. This study recorded the membrane potentials of multiple hippocampal CA1 pyramidal cells in mice. For this study, whole-cell recordings were obtained from Slc:ICR mice, PV-Cre mice were used for optogenetical manipulation, and C57BL/6J mice were used for chronic extracellular recordings. The dataset contains electrophysiology and immunohistochemistry data. The data showed that pre-SWR inhibitory activity determines the sequential spike times of pyramidal cells and diversifies the repertoire of sequence patterns.