Description

This study showed that basal-like breast cancer (BLBC) is sensitive to suppression of iron-sulfur cluster (ISC) biosynthesis and identified DNA polymerase epsilon (POLE) as an ISC-containing protein that underlies this phenotype. This dataset includes RNA sequencing data and data underlying figures. The publication also contains supplementary data, which includes data for short hairpin RNA (shRNA) information, shRNA knockdown efficacy, and normalized population doublings upon shRNA expression relative to non-targeting shRNA, correlations between ISCU suppression sensitivity to suppression sensitivity of other genes across CCLE breast cancer cell lines, correlation of POLE sensitivity with gene expression in CCLE cell lines, RNA sequencing data for MDA-MB-231, CAMA-1, and MCF7 cells expressing shRNAs targeting POLE and either a vector control or cDNA POLE rescue, and primary phosphoproteomic data.

Subject of Study
Subject Domain
Keywords

Access

Restrictions
Free to All
Instructions
RNA sequencing data are available at Gene Expression Omnibus (GEO) and data underlying figures can be accessed at Mendeley Data. All other data are available on PubMed Central (PMC) under Supplementary Material.
Access via GEO

RNA sequencing data
Accession #: GSE159052

Access via Mendeley Data

Data underlying figures

Access via PMC

Other data
Accession #: PMC7687292

Associated Publications
Data Type
Equipment Used
Allegra X-12R Benchtop Centrifuge
Beckman Coulter Z2
Heracell 150i Incubator
Zeiss AxioImager M1
Software Used
FlowJo v10
ImageJ
Matplotlib
NumPy
pandas
SciPy
Seaborn
Grant Support
Pew Biomedical Scholars/Pew Charitable Trusts
Alexander and Margaret Stewart Trust/Alexander and Margaret Stewart Trust
ACS Research Scholar Grant/American Cancer Society
Susan G. Komen for the Cure/Breast Cancer Foundation