Ultraviolet radiation (UVR) from the sun is the major cause of skin cancer. However, genes and pathways altered by solar-simulated UVR (ssUVR), a mixture of UVA and UVB, still remains unclear. For this study, human HaCaT keratinocytes were exposed to a single dose or 5 repetitive doses of ssUVR. Analyses of gene expression profiles and functional annotation were performed at 24 hours after irradiation. This study contains RNA sequencing data. The results showed that ssUVR modified genes with diverse cellular functions in a dose-dependent manner and gene expression in cells exposed to a single dose of ssUVR was notably different from those that underwent repetitive exposures.