Transposon Insertion Profiling by Sequencing (TIPseq) Utilized for LINE-1 Mapping in Single Cells
- Description
This dataset was compiled to determine a genome-wide long interspersed element-1 (LINE-1) insertion site mapping method for bulk DNA. This study has shown that transposon insertion profiling by sequencing (TIPseq) is a method that can be used for LINE-1 mapping in single cells. For the study, live GM12878 lymphoblastoid cells were obtained from Coriell Institute for Medical Research. The dataset contains sequencing data. The publication also includes supplementary data. The supplementary data contains one table about primers for quality control qPCR. It also contains another table about multiple displacement amplification or multiple annealing and looping-based amplification cycles, which was used to perform single-cell whole-genome amplification.
Access
- Restrictions
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Free to All
- Instructions
- All the sequencing data are deposited at NCBI Sequence Read Archive (SRA) with BioProject. The analyzed data supporting this article are present in the Supplementary Materials.
- Grant Support