NYU Dataset

Transposon Insertion Profiling by Sequencing Utilized for LINE-1 Mapping in GM12878 Cells

Part of: Fenyo Lab |
UID: 10460
* Corresponding Author
Description

This study reported a genome-wide long interspersed element-1 (LINE-1) insertion site mapping method for bulk DNA. They demonstrated that transposon insertion profiling by sequencing (TIPseq) is useful for LINE-1 mapping in GM12878 cells. In addition, they evaluated the performance of different procedures and compared them to TIPseq in bulk DNA. This dataset contains sequencing data as well as supplementary data tied to the publication. The supplementary data includes data on primers for quality control quantitative polymerase chain reaction and multiple displacement amplification or multiple annealing and looping-based amplification cycles.

Subject of Study
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Keywords

Access

Restrictions
Free to All
Instructions
The sequencing data are deposited to Sequence Read Archive (SRA) through BioProject. All other data are present in the article on PubMed Central (PMC) under Supplementary Material.
Access via BioProject

Sequencing data
Accession #: PRJNA547805

Access via PMC

Supplementary data
Accession #: PMC7061987

Associated Publications
Data Type
Equipment Used
Illumina HiSeq 4000
Software Used
TIPseqHunter2
Grant Support