Peripheral Blood Leukocytes Gene Expression Predicts Risk for Progression of Symptomatic Knee Osteoarthritis
- Description
This study investigated gene expression profiles in peripheral blood leukocytes of patients with osteoarthritis (OA) in comparison with non-OA controls to evaluate whether gene expression profiles could serve as biomarkers of symptomatic knee OA. Three cohorts were studied: two cohorts (learning cohort and validation cohort) recruited at New York University Hospital for Joint Diseases (NYUHJD) and one (validation cohort) at Duke University Medical Center. The NYUHJD Learning Cohort recruited 45 OA patients and 25 non-OA controls. Five microgram of total RNA isolated from peripheral blood mononuclear cell (PBMC) was used for biotin cRNA synthesis and hybridized against Affymetrix human U133A chip. In addition, microarray assessment was accomplished for 41 OA patients and 25 non-OA controls. Using Significance Analysis of Microarrays, they identified 173 genes significantly up-regulated or down-regulated in the NYUHJD Learning Cohort OA PBMC. Two additional cohorts, NYUHJD Validation Cohort and Duke Validation Cohort, were used for validation of IL-1β expression.
- Geographic Coverage
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New York (State) - New York CityNorth Carolina - Durham
Access
- Restrictions
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Free to All
- Instructions
- Microarray data are available in Gene Expression Omnibus (GEO).
- Grant Support