Targeting of Intracellular Oncoproteins with Peptide-Centric Chimeric Antigen Receptors
- Description
This study demonstrated that the neuroblastoma immunopeptidome is enriched with peptides derived from proteins essential for tumorigenesis. They targeted the unmutated peptide QYNPIRTTF discovered on HLA-A*24:02, which is derived from the neuroblastoma-dependency gene and master transcriptional regulator PHOX2B. To target QYNPIRTTF, they developed peptide-centric chimeric antigen receptors (PC-CARs) through a counter panning strategy using predicted potentially cross-reactive peptides. This dataset includes neuroblastoma HLA class I immunopeptidomics data, protein structures, and immunohistochemistry data. The results show that PC-CARs have the potential to expand immunotherapeutic targets to include non-immunogenic intracellular oncoproteins and allow targeting through additional HLA allotypes.
Access
- Restrictions
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Free to All
- Instructions
- Neuroblastoma HLA class I immunopeptidomics data are available through Proteomics Identifications Database(PRIDE) and the protein structures are available in the Protein Data Bank (PDB). All other data are available within the article on PubMed Central (PMC) under Supplementary Materials.
- Grant Support
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SU2C-AACR-DT-27-17/American Association for Cancer ResearchScience Center Quod Erat Demonstrandum/Philadelphia Science CenterCell and Gene Therapy Collaborative/Children’s Hospital of PhiladelphiaGiulio D’Angio Endowed Chair in Neuroblastoma Research/Children's Hospital of PhiladelphiaDE-AC02-05CH11231/United States Department of Energy
- Other Resources
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sCRAP
Algorithm