NYU Dataset

Dietary Thiols Accelerates Aging of Caenorhabditis elegans

Part of: Nudler Lab |
UID: 10645
* Corresponding Author
Description

Glutathione (GSH) and its precursor, N-acetylcysteine (NAC), are common thiol antioxidants, which are among the most popular dietary supplements. However, the long-term effects of GSH or NAC on healthy animals have not been properly studied. This study examined the effects of prolonged supplementation and chronic restriction of GSH and NAC on Caenorhabditis elegans physiology and lifespan. For RNA interference (RNAi) experiments, eggs were isolated and allowed to develop and grow for two generations on specific RNAi expressing bacterial strains before being used for lifespan analyses. This dataset contains RNA sequencing data. The data indicates that it is important for healthy individuals to avoid excessive dietary antioxidants.

Subject of Study
Subject Domain
Keywords

Access

Restrictions
Free to All
Instructions
RNA sequencing data generated in this study have been deposited to NCBI's BioProject. Source data are provided with this paper on PubMed Central (PMC) under Supplementary Materials and code for RNA sequencing data processing and differential expression analysis is available on GitHub.
Access via BioProject

RNA-seq data
Accession #: PRJNA714646

Access via PMC

Source data
Accession #: PMC8282788

Associated Publications
Data Type
Equipment Used
Illumina NextSeq 500
Thermo Scientific EVOS
Zeiss Axio Zoom.V16
Software Used
cummeRbund v2.0
DESeq2 v1.10.0
GeneCodis3
HISAT2 v2.1.0
HTSeq v0.11.0
ImageJ
Microsoft Excel
Nematode Bioinformatics
R v2.15.1
SciDAVis
WormBase
ZEN
Grant Support
Howard Hughes Medical Institute/Howard Hughes Medical Institute
Blavatnik Family Foundation/Blavatnik Family Foundation
19-74-30007/Russian Science Foundation
17-74-30030/Russian Science Foundation
Glenn Foundation for Medical Research/Glenn Foundation for Medical Research