Learning and memory are presumed to be supported by mechanisms that involve cholinergic transmission and hippocampal theta. This study examined the effect of sustained cholinergic activation on memory performance. For this study, they used G protein-coupled receptor activation based acetylcholine sensor to monitor acetylcholine activity during memory performance in mice. The dataset contains behavioral, electrophysiology, and immunohistochemistry data. The study showed enhanced cholinergic activation in the hippocampus during the delay between choices in a spontaneous alternation task abolished sharp-wave ripples (SPW-Rs) and impaired choice performance in mice.