Adenovirus is a nuclear replicating DNA virus that relies on the host cell machinery for productive infection. Processing and metabolism of cellular RNAs are regulated by METTL3, which catalyzes the addition of N6-methyladenosine (m6A) to messenger RNAs. Although m6A-modified adenoviral RNAs have been detected previously, the location and function of m6A within the infectious cycle is unknown. For this study, they examined m6A within the adenovirus transcriptome using a combination of methylated RNA immunoprecipitation (meRIP) sequencing and direct RNA long-read sequencing.

This study includes sequencing data. The publication also contains supplementary data that includes several tables. The tables contain data about sequencing statistics for meRIP and RNA sequencing experiments, meRIP sequencing derived MACS2 peak locations for mock-infected cellular genes, Ad5-infected cellular genes, viral genes, and peak widths, direct RNA sequencing statistics for experiments, locations of exome-level direct RNA sequencing analysis of m6A locations within viral transcripts, and locations of isoform-level direct RNA sequencing analysis of m6A locations within viral transcript isoforms.