NYU Dataset

Sequencing Data for a Delta-Omicron Recombinant in an Immunosuppressed Transplant Recipient Treated with Monoclonal Antibody Sotrovimab

UID: 10454
* Corresponding Author
Description
A Delta-Omicron recombinant of the SARS-CoV-2 virus was sequenced and isolated from an immunosuppressed and unvaccinated male kidney transplant patient who received Sotrovimab for treatment of COVID-19. Further clinical details and medical history of the patient are described in the associated publication. Sotrovimab is a therapeutic monoclonal antibody (mAb) used to treat infections by the BA.1 Omicron lineage. It was observed that the Delta-Omicron recombinant found in this patient was resistant to neutralization by Sotrovimab. Genomic analysis of the SARS-CoV-2-positive nasopharyngeal swab specimen revealed an unusual SARS-CoV-2 sequence with outlier Omicron BA.1 placement in a global phylogenetic tree and high number of mutations.
Timeframe
2022
Subject of Study
Subject Domain
Keywords

Access

Restrictions
Free to All
Application Required
Instructions
A GISAID account is required to access the sequencing data shared in the GISAID repository. Users must complete a Database Access Agreement and Registration Form to obtain credentials. Raw FASTQ files have been shared in the Sequence Read Archive (SRA), accessible through BioProject.
Access via GISAID

Virus sequencing data
Accession #: EPI_ISL_10792641

Access via BioProject

Raw sequence reads
Accession #: PRJNA915368

Associated Publications
Data Type
Equipment Used
bioMerieux NucliSENS easyMAG
Illumina NovaSeq 6000
Thermo Scientific Ion S5 XL
Software Used
bcl2fastq v2.20
BEAST v1.10.5
BioRender
ChimeraX v1.3
Freyja
GraphPad Prism v8.0
IQ-TREE v2.2.0
Nextclade v1.14
Nextstrain CLI v6.0.0
Pangolin v3.1.19
Sambamba v0.6.8
Trimmomatic v0.36
Study Type
Observational
Dataset Format(s)
FASTQ
Grant Support
G0E1420N/Research Foundation - Flanders
G098321N/Research Foundation - Flanders
C14/18/094/Internal Funds KU Leuven
NU50CK000516/CDC
V420922N/Research Foundation Flanders
Jan Vilcek/David Goldfarb Fellowship Endowment Fund/NYU Langone Health
Other Resources
ncov2019-artic-nf Pipeline

Consensus genome

SWISS-MODEL

Fully automated protein structure homology-modelling server

UShER

Ultrafast Sample placement on Existing tRee