NYU Dataset

Probing Subthreshold Dynamics of Hippocampal Neurons by Pulsed Optogenetics

Part of: Buzsaki Lab |
UID: 10679
* Corresponding Author
Description

This study investigated the subthreshold dynamics using optogenetic depolarizing pulses in hippocampal neuronal assemblies in freely moving mice to understand how excitatory and inhibitory inputs are integrated by neurons. For the study, they recorded and examined large numbers of CA1 pyramidal neurons simultaneously in freely moving CamKIIα-Cre::Ai32 mice. This dataset contains electrophysiology and imaging data. These results indicate that indirect probing of subthreshold dynamics in neuronal populations permits the disclosing of preexisting assemblies and modes of neuronal operations.

Subject of Study
Subject Domain
Keywords

Access

Restrictions
Free to All
Instructions
All data needed to evaluate the conclusions in the paper are publicly available in the Buzsaki Lab Webshare and custom MATLAB scripts can be downloaded from GitHub.
Associated Publications
Data Type
Equipment Used
Basler acA1300-60gmNIR
Intan RHD2000 Evaluation System
Leica Leitz DMRB
Leica VT1000 S
Molecular Devices Axon Axoclamp 900A
Sutter MP-285
Sutter P-97
Zeiss Confocal Microscope
Software Used
Kilosort
KilosortWrapper
MATLAB
Phy
Phy Plugins
Grant Support
1707316/NSF
Leon Levy Fellowship in Neuroscience/Leon Levy Foundation
1545858/NSF
LT0000717/2018/Human Frontiers Science Program
EMBO ALTF 1161-2017/European Molecular Biology Organization