Elongin A Regulates Transcription In Vivo Through Enhanced RNA Polymerase Processivity
- Description
Elongin, an RNA polymerase II (RNAPII)-associated factor, has shown to stimulate transcriptional elongation in vitro. The Elongin complex is assumed to be required for transcriptional induction in response to cellular stimuli. However, the role of this complex during transcription has not been studied in vivo. This study performed comprehensive studies of the role of Elongin A, the largest subunit of the Elongin complex, on RNAPII transcription genome-wide. The dataset contains RNA sequencing, 4-thiouridine sequencing, and ChIP sequencing data. In addition, the dataset contains mass spectrometry data. The results indicate that unlike in vitro findings, depletion of Elongin A has little impact on global transcription profiles and transcription elongation in vivo.
Access
- Restrictions
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Free to All
- Instructions
- All next-generation sequencing data have been deposited to Gene Expression Omnibus (GEO). Mass spectrometry raw files are accessible under MassIVE and ProteomeXchange.
- Grant Support
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Research Start-Up Funding/Nanjing Agricultural University