NYU Dataset

Fluoride Exposure Alters Calcium Signaling and Mitochondrial Function in Enamel Cells

Part of: Lacruz Lab |
UID: 10636
* Corresponding Author
Description

Fluoride is a naturally occurring mineral that helps build strong teeth and prevent cavities. However, excessive fluoride intake causes dental fluorosis, which are enamel defects that can increase the risk of tooth decay. This study examined the effects of fluoride exposure in enamel cells to assess its impact on calcium signaling. For their investigation, they isolated primary EO cells from Sprague Dawley rats. They also used LS8 cells and HEK293 cells because primary EO cells are difficult to maintain in culture. The dataset includes RNA sequencing data. These data provide a potential mechanism underlying dental fluorosis.

Subject of Study
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Access

Restrictions
Free to All
Instructions
RNA sequencing data have been deposited in NCBI's Gene Expression Omnibus (GEO) database. All other data needed to evaluate the conclusions in the paper are available on PubMed Central (PMC) under Supplementary Materials.
Access via GEO

RNA sequencing data
Accession #: GSE143536

Access via PMC

Other data
Accession #: PMC7173621

Associated Publications
Data Type
Equipment Used
Agilent 2200 TapeStation System
Agilent Seahorse XFe24 Analyzer
BD LSR II
Bio-Rad CFX Connect
Bio-Rad ChemiDoc MP
Illumina NovaSeq 6000
Molecular Devices FlexStation 3
Nikon Eclipse TE2000
Nikon Eclipse TE2000 U
Qubit 2.0 Fluorometer
Software Used
Cuffdiff v2.2.1
Cufflinks v2.2.1
FACSDiva
FastQC
FlowJo
GraphPad Prism v7.0
JMP Genomics v8
STAR v2.5.2a
Trimmomatic v0.32
Grant Support