Oxytocin is a neuropeptide released in the central nervous system, which is involved in a wide range of behaviors including reproduction, parental care, and pair bonding. In addition, peripheral oxytocin is important for milk ejection during nursing and uterine contractions during labor. Oxytocin is produced mainly in the paraventricular nucleus (PVN) and supraoptic nucleus of the hypothalamus. This study describes a neural circuit routing auditory information about infant vocalizations to mouse oxytocin neurons. They performed in vivo electrophysiological recordings and photometry from identified oxytocin neurons in awake maternal mice presented with pup calls. For this study, they made cell-attached and whole-cell recordings from PVN oxytocin neurons and other optically-unresponsive PVN neurons in awake head-fixed mouse dams. The dataset contains electrophysiology, behavioral, histology, and fiber photometry data. This study reveals a circuit that unlocks central oxytocin release and maternal behavior in response to pup calls.