NYU Data Catalog

NYU Dataset

Lateral Entorhinal Cortex Inputs Modulate Hippocampal Dendritic Excitability

Part of: Basu Lab |
UID: 10608
Author(s): Olesia M. Bilash, Spyridon Chavlis, Cara D. Johnson, Panayiota Poirazi, Jayeeta Basu*
* Corresponding Author
Description

Lateral entorhinal cortex (LEC) performs important functions for episodic memory processing and coding for contextually salient elements of an environment or experience. This study combined functional circuit mapping and computational modeling to examine the cellular and circuit mechanisms by which long-range glutamatergic LEC projections influence the local activity in the hippocampus. This dataset contains electrophysiology, immunohistochemistry, and modeling data. The data indicates a cortically driven GABAergic microcircuit mechanism that gates nonlinear dendritic computations, which supports compartment-specific coding of multisensory contextual features within the hippocampus.

Subject of Study
Ai14 mice
Ai80D mice
CaMKII-Cre mice
VIP-IRES-Cre mice
SST-IRES-Cre mice
CCK-IRES-Cre mice
CR-IRES-Cre mice
VIP-IRES-Flp mice
Dlx-Flp mice
Ai65D mice
Subject Domain
Neuroscience
Keywords
Animal Model
Animals
Brain
Dendrites
Electrophysiology
Entorhinal Cortex
Hippocampus
Immunohistochemistry
Memory
Neurons
Neurosciences
Optogenetics
Pyramidal Cells

Access

Restrictions
Application Required
Author Approval Required
Instructions
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Associated Publications
Bilash OM, Chavlis S, Johnson CD, Poirazi P, Basu J. Lateral entorhinal cortex inputs modulate hippocampal dendritic excitability by recruiting a local disinhibitory microcircuit. Cell Rep. 2023 Jan 31;42(1):111962.
Data Type
Electrophysiological
Imaging
Equipment Used
Digitimer DS8R

Biphasic constant current stimulator

Drummond Scientific Nanoject II

Auto-nanoliter injector

In-line Solution Heater

Warms solutions flowing into imaging and recording chamber

Leica VT1000 S

Vibrating blade microtome

Leica VT1200 S

Automated vibrating blade microtome

LN Mini Micromanipulator

Performs patch-clamp recordings

LNscope Optical Microscope

Imaging platform

Matrx VIP 3000 Vaporizer

Veterinary anesthetic TEC 3-style vaporizer

Molecular Devices MultiClamp 700B

Microelectrode amplifier

ORCA-R2 CCD Camera

Camera for quantitative imaging

Sutter P-1000

Next generation micropipette puller

Thorlabs LED System

Performs patch-clamp recordings

Zeiss LSM 520 META

Confocal microscope

Software Used
Adobe Illustrator

Designs custom graphics and illustrations

AxoGraph v1.7.0

Provides professional-quality graphs, charts, and figures

CentOS v7.9

Community-supported computing platform

GraphPad Prism

Statistical analysis

ImageJ

Java based image processing

MATLAB R2019b

Programming and numeric computing platform

NEURON v8.0.0

Computational models and networks of neurons

pCLAMP v9

Data acquisition and analysis software

Python v3.9.7

Programming language

ZEN

Software for image acquisition and analysis

μManager

Software for control and automation of microscope hardware

Grant Support
Alfred P. Sloan Foundation/Alfred P. Sloan Foundation
Whitehall Foundation/Whitehall Foundation
Mathers Foundation/Mathers Foundation
P30 AG066512/NIA NIH
Klingenstein-Simons Foundation/Klingenstein-Simons Foundation
T32 NS086750/NINDS NIH
R01 NS109994/NINDS NIH
R01 NS109362/NINDS NIH
McKnight Foundation/McKnight Foundation
American Epilepsy Society/American Epilepsy Society
Blas Frangione Foundation/Blas Frangione Foundation
NYU Whitehead Fellowship/NYU Whitehead Fellowship
GA 863254/European Commission
EVF-2019-508/Einstein Foundation Berlin
Leon Levy Foundation/Leon Levy Foundation
R01 MH124867/NIMH NIH