Proper Mitochondrial Translation Initiation and Maintenance of Formylmethionyl tRNAs Requires SHMT2
- Description
Eukaryotic cells upregulate oxidative metabolism to maintain homeostasis upon glucose restriction. This study used CRISPR/Cas9-based screening to identify serine catabolic enzyme SHMT2. They found that the mitochondrial SHMT2 is required for robust mitochondrial oxygen consumption and low glucose proliferation. This dataset contains immunoblot images, as well as supplementary data tied to the publication. The supplementary data includes tables, which contains data for high versus low glucose primary screening, which is the primary screening data for the sgRNA based-screen, and oligonucleotides used in this study. This study shows that SHMT2 loss impacts mitochondrial translation, which depletes mitochondrially encoded proteins and decreases respiration.
Access
- Restrictions
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Free to All
- Instructions
- Primary data are available within the article on PubMed Central (PMC) under Supplementary Materials. Immunoblot images has been uploaded to Mendeley Data.
- Grant Support
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Pew-Stewart Scholars for Cancer Research/Pew Charitable TrustsIrma T. Hirschl Trust/Irma T. Hirschl TrustSusan G. Komen for the Cure/Breast Cancer FoundationV Foundation/V FoundationAACR NextGen Grant/American Association for Cancer ResearchHoward Hughes Medical Institute/Howard Hughes Medical InstituteSearle Scholar/Kinship FoundationSidney Kimmel Scholar/Sidney Kimmel FoundationBasil O’Connor Starter Scholar/March of Dimes