Cortical inhibitory and excitatory inputs are initially mismatched, but become balanced with experience during development. However, how excitatory-inhibitory balance is defined at most synapses, or the mechanisms for maintaining this balance at specific set-points is still unclear. This study demonstrated how coordinated long-term plasticity calibrates populations of excitatory/inhibitory inputs onto mouse auditory cortical pyramidal neurons. C57BL/6 mice of both sexes were used in all experiments. For this study, acute slices of auditory cortex were prepared from juvenile and adult C57Bl/6 mice, which included the age range spanning the critical period for excitatory-inhibitory balancing in rodent auditory cortex. The dataset includes electrophysiology and two-photon calcium imaging data.