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NYU Dataset

ORF2p Eludes Detection in Mass Spectrometry-Based Tumor Proteome Profiling

Part of: Fenyo Lab |

UID: 10462

Author(s): Daniel Ardeljan, Xuya Wang, Mehrnoosh Oghbaie, Martin S. Taylor, David Husband... See more...

Vikram Deshpande, Jared P. Steranka, Mikhail Gorbounov, Wan Rou Yang, Brandon Sie, H. Benjamin Larman, Hua Jiang, Kelly R. Molloy, Ilya Altukhov, Zhi Li, Wilson McKerrow, David Fenyo*, Kathleen H. Burns, John LaCava

* Corresponding Author

Description: This study showed that long interspersed element-1 (LINE-1) ORF2p expression is almost undetectable in human cancers. LINE-1 is the major driver of mobile DNA activity in humans. When expressed, LINE-1 loci produce bicistronic transcripts encoding two proteins essential for retrotransposition, ORF1p and ORF2p. ORF2p expression is not well characterized in human tissues and cell lines. For the study, HEK-293TLD cells were used to examine ORF2p expression. This dataset includes mass spectrometry proteomics data as well as supplementary data tied to the publication. The supplementary data contains data on antibody epitope matches for 31 'hot' human-specific LINE-1 loci and summary of the mass spectrometry based proteomic results.

Subject of Study

Subject Domain

Keywords

Mass Spectrometry

Open Reading Frames

Phosphorylation

Access

Restrictions: Free to All
Instructions: The mass spectrometry proteomics data have been deposited to the PRoteomics IDEntifications (PRIDE) database.; Access via PRIDE
Mass spectrometry proteomics data
Accession #: PXD013743

Associated Publications

Ardeljan D, Wang X, Oghbaie M, Taylor MS, Husband D, Deshpande V, Steranka JP, Gorbounov M, Yang WR, Sie B, Larman HB, Jiang H, Molloy KR, Altukhov I, Li Z, McKerrow W, Fenyö D, Burns KH, LaCava J. LINE-1 ORF2p expression is nearly imperceptible in human cancers. Mob DNA. 2019 Dec 31;11:1.

Data Type

Equipment Used

Illumina HiSeq 2500

LI-COR Odyssey Scanner

Thermo Scientific Q Exactive Plus

Zeiss Confocal Microscope

Software Used

Line 1 CRC IP MS

MaxQuant

ProteoWizard

Grant Support: R01 GM124531/NIGMS NIH

P41 GM109824/NIGMS NIH

UL1 TR001866/NCATS NIH

F30 CA221175/NCI NIH

R01 GM126170/NIGMS NIH

19–0223/Worldwide Cancer Research

R01 GM130680/NIGMS NIH

P50 GM107632/NIGMS NIH

U24 CA210972/NCI NIH

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