ORF2p Eludes Detection in Mass Spectrometry-Based Tumor Proteome Profiling
- Description
This study showed that long interspersed element-1 (LINE-1) ORF2p expression is almost undetectable in human cancers. LINE-1 is the major driver of mobile DNA activity in humans. When expressed, LINE-1 loci produce bicistronic transcripts encoding two proteins essential for retrotransposition, ORF1p and ORF2p. ORF2p expression is not well characterized in human tissues and cell lines. For the study, HEK-293TLD cells were used to examine ORF2p expression.
The dataset includes mass spectrometry proteomics data. The publication also has supplementary data that includes two tables. First table contains antibody epitope matches for 31 'hot' human-specific LINE-1 loci. For each locus, the study specifies whether the ORF2p sequence is identical to the antibody epitope or whether it differs from the epitope. The Brouha BAC clone designation, activity rating, and chromosomal positions are also included in the table. Second table contains summary of the mass spectrometry based proteomic results, which includes identified and statistically significant proteins, proteins observed in other studies, ORF1p loci detected, and phosphorylation of S18 and S27 peptide spectrum matches.
Access
- Restrictions
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Free to All
- Instructions
- The mass spectrometry proteomics data have been deposited to the ProteomeXchange Consortium via the Proteomics Identifications (PRIDE) partner repository.
- Grant Support
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19–0223/Worldwide Cancer Research