-
NYU Dataset
Transposon Insertion Profiling by Sequencing Utilized for LINE-1 Mapping in GM12878 Cells
- Authors
- Wilson McKerrowZuojian TangJared P. SterankaLindsay M. Payer5 more author(s)...
- Description
This study reported a genome-wide long interspersed element-1 (LINE-1) insertion site mapping method for bulk DNA. They demonstrated that transposon insertion profiling by sequencing (TIPseq) is useful for LINE-1 mapping in GM12878 cells. In addition, they evaluated the performance of different procedures and compared them to TIPseq in bulk DNA. This dataset contains sequencing data as well as supplementary...
- Subject
-
CancerGenomics
- Access Rights
- Free to All
-
NYU Dataset
Condensation of ORF1p is Critical for LINE-1 Retrotransposition
- Authors
- Srinjoy SilSarah KeeganFarida EttefaLance T. Denes2 more author(s)...
- Description
Long interspersed element-1 (LINE-1) is an autonomously active retrotransposon in the human genome. The LINE-1 mRNA encodes two proteins, ORF1p and ORF2p, which are essential for retrotransposition. This study showed that electrostatic interactions and trimer conformational dynamics together tune the properties of ORF1p assemblies to allow for efficient LINE-1 ribonucleoprotein complex formation in...
- Subject
-
Cell Physiology
- Access Rights
- Free to All