This study used proteomic analysis, respirometry, and electron microscopy to examine the development of condensed mitochondria. The dataset contains proteomic and imaging data. The data indicate that condensed mitochondria formed from orthodox mitochondria during meiosis in order to support the formation of the acrosomal matrix. Condensed mitochondria also respired more actively and contained more proteins involved in protein import compared to orthodox mitochondria. The data also showed that the abundance of condensed mitochondria declined after the completion of meiosis.