Protein Modifications Discovered Using Differential Tandem Mass Spectrometry Proteomics
- Description
This dataset was collected to present a spectral alignment method for the identification of protein modifications using high-resolution mass spectrometry proteomics. For this study, they used SAMPEI for spectral alignment-based modified peptide identification. SAMPEI is an open-source algorithm that is designed for the discovery of functional protein and peptide signaling modifications. Human OCI-AML2 and mouse RAW264.7 cells were used to discover protein modifications. This study includes mass spectrometry data as well as supplementary data tied to the publication. The supplementary data contains one table about list of chemically modified peptides agnostically identified using SAMPEI in the proteome of LPS stimulated RAW264.7 cells. It also contains another table about list of peptides with putative itaconate adducts identified in RAW264.7 proteomes using variable modification search.
Access
- Restrictions
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Free to All
- Instructions
- Mass spectrometry data have been deposited to the ProteomeXchange Consortium via the PRIDE partner repository and processed files are available from Zenodo.
- Grant Support
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St. Baldrick’s Foundation/St. Baldrick’s FoundationHyundai Hope On Wheels/Hyundai Hope On WheelsCareer Awards for Medical Scientists/Burroughs Wellcome FundRita Allen Foundation/Rita Allen FoundationLeukemia & Lymphoma Society/Leukemia & Lymphoma SocietyStarr Cancer Consortium/Starr Cancer ConsortiumCenter for Experimental Therapeutics/MSKCCDamon Runyon Cancer Research Foundation/Damon Runyon Cancer Research FoundationRichard Lumsden Foundation/Richard Lumsden FoundationPershing Square Foundation/Pershing Square FoundationMathers Foundation/Mathers FoundationCommonwealth Foundation for Cancer Research Foundation/Commonwealth Foundation for Cancer Research Foundation