Mass Spectrometry Data Suggests Mitovesicles are Altered in Down Syndrome
- Description
This dataset showed that mitovesicles, extracellular vesicles (EVs) of mitochondrial origin, are altered in Down syndrome (DS). Mitochondrial dysfunction is a distinctive feature of aging and neurodegenerative disorders, such as DS and Alzheimer’s disease. For this study, a high-resolution density gradient separation of EVs isolated from murine and human DS and diploid control brains was used. Using this method, they identified and characterized previously unknown population of double-membraned EVs. The double-membraned EVs contained multiple mitochondrial proteins different from formerly described EV subtypes, including microvesicles and exosomes.
The dataset contains mass spectrometry data. The publication also has supplementary data that includes several tables. The tables contain data about density range of the fractions obtained by the OptiPrep step-gradient used to separate brain EVs, mitochondrial proteins found in fraction 8 and clustered by function, proteins present only in 2N mice EVs, proteins that are more abundant in 2N mice than in Ts2 mice EVs, proteins present only in Ts2 mice EVs, and proteins that are more abundant in Ts2 mice than in 2N mice EVs. The tables also contain data about gene ontology analysis of the top 50 differentially expressed pathways in Ts2 mice EVs compared to diploid littermates and specification of the human subjects whose frozen Broadmann area 9 (BA9) samples were used for EV isolation.
Access
- Restrictions
-
Free to All
- Instructions
- The mass spectrometry data for this study are available at MassIVE. The supplementary materials shared with the associated publication include tables of detected proteins and pathways, and resource lists.
- Grant Support