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  • NYU Dataset

    Structural Basis of Ion-Substrate Coupling in the Sodium-Dependent Dicarboxylate Transporter VcINDY

    Authors
    David B. Sauer
    Jennifer J. Marden
    Joseph C. Sudar
    Jinmei Song
    2 more author(s)...
    Description

    VcINDY is a sodium-dependent dicarboxylate transporter that imports tricarboxylic acid cycle intermediates across the inner membrane of Vibrio cholerae. This study used a combination of structure determination by single-particle cryo-electron microscopy, substrate-binding affinity measurements by intrinsic tryptophan fluorescence quenching, and position accessibility quantification by site-directed...

    Subject
    Proteomics
    Access Rights
    Free to All
  • NYU Dataset

    Unravelling Cysteine-Deficiency-Associated Rapid Weight Loss

    Authors
    Alan Varghese
    Ivan Gusarov
    Begona Gamallo-Lana
    Daria Dolgonos
    13 more author(s)...
    Description

    The growing interest in diets that induce weight loss prompted this study to compare restriction of each individual essential amino acid (EAA) and cysteine. For the study, each of the essential amino acids (EAAs) and cysteine were individually removed from diet in Cse−/− and Cse+/− mice to induce weight loss. To evaluate the molecular responses triggered by cysteine deprivation, bulk RNA-sequencing...

    Subject
    Genomics
  • NYU Dataset

    Mechanism of Beta-Arrestin 1 Mediated Src Activation via Src SH3 Domain

    Authors
    Natalia Pakharukova
    Brittany N. Thomas
    Harsh Bansia
    Linus Li
    12 more author(s)...
    Description

    Beta-arrestins (βarrs) are key transducers of G-protein coupled receptors, but the mechanisms of βarr signaling are poorly understood. Therefore, this study examined the structural mechanisms underlying βarr-mediated signal transduction. Using cryo-electron microscopy (cryo-EM), the study examined how βarr1 recruits and activates the non-receptor tyrosine kinase Src, a well-established signaling partner...

    Subject
    Proteomics
    Access Rights
    Free to All