Previous studies showed the potential of calreticulin (CRT) protein in enhancing the rate and quality of wound healing in excisional animal wound models. This study determined if polycaprolactone (PCL)/type 1 collagen (Col1) nanofibers (NFs) could be used as an effective carrier for CRT while at the same time introducing further wound healing capability through the synergy of biological and physicochemical cues. For the study, they used human keratinocytes and fibroblasts. This dataset contains cell proliferation, cell mobility, wound-gap closure, immunofluorescent, and western blot data. The data reveal that CRT electrospun into PCL/Col1 NFs retains its structural integrity and biological functions.