NYU Dataset

Synaptic Vesicle Traffic is Supported by Transient Actin Filaments

Part of: Tsien Lab |
UID: 10561
* Corresponding Author
Description

Synaptic vesicles (SVs) play a key role in synaptic transmission. This study used time-lapse microscopy to demonstrate two distinct mechanisms of vesicle traffic along axons. They employed specific pharmacological interventions along with imaging of transient actin polymerization and traffic of recycling SVs loaded with single quantum dots to characterize the underlying axonal actin structures. For the study, hippocampal neurons were cultured from postnatal day 0 male and female Sprague Dawley rat pups. The dataset contains time-lapse imaging of vesicle traffic and actin polymerization, and immunocytochemistry data.

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Equipment Used
Grass SD9 B Square Pulse Stimulator
Nikon Eclipse Ti-S
Zeiss LSM 800