Human Oral Microbiota Composition Data from UAE Healthy Future Study (UAEHFS) Pilot Participants
- Description
This dataset includes 330 Emirati adults participating in the UAE Healthy Future Study-Pilot (UAEHFS-pilot) who provided mouthwash samples. There were 225 non-smokers and 105 smokers who used cigarettes, dokha, shisha, or a combination of tobacco products. 101 of the 105 smokers were male.
Tobacco use and frequency were assessed with a structured questionnaire and urine cotinine lab results. Gene sequencing was performed on the bacterial 16S rRNA gene from mouthwash samples. The investigators amplified the DNA from the V4 region (515 F-5′GTGCCAGCMGCCGCGGTAA3′ - 806 R - 5′GGACTACHVGGGTWTCTAAT3′). The amplified samples were prepared in agar gel and purified, then further sequencing was performed in two batches with 3 quality control samples. A detailed methodology is provided in the associated publication. In total, 16,132,922 high quality 16S rRNA sequences were obtained.
Bacteria from the following phyla were detected in the oral samples (listed in descending order of abundance):
- Firmicutes
- Bacteroidetes
- Proteobacteria
- Actinobacteria
- Fusobacteria
- Spirochaetes
- SR1
- Synergistetes
- Tenericutes
- Cyanobacteria
- GN02
- TM7
- Chloroflexi
- Timeframe
- 2014 - 2015
- Geographic Coverage
-
United Arab Emirates
Access
- Restrictions
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- Instructions
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