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NYU Dataset
PIM1 Phosphorylation Regulates Gene Transcription in Prostate Cancer
- Authors
- Sophie E. RuffNikita VasilyevEvgeny NudlerSusan K. Logan1 more author(s)...
- Description
Previous study showed that PIM1 phosphorylates the androgen receptor (AR), the primary therapeutic target in prostate cancer. This study examined the mechanism how PIM1 phosphorylation of AR alters its transcriptional activity. They identified the AR co-activator, 14-3-3 ζ, as an endogenous PIM1 substrate in LNCaP cells. Rapid immunoprecipitation and mass spectrometry of endogenous proteins on chromatin...
- Subject
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CancerGenomicsProteomics
- Access Rights
- Free to All
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NYU Dataset
Identification of PIM1 Substrates Reveals a Role for NDRG1 Phosphorylation in Prostate Cancer
- Authors
- Russell J. LedetSophie E. RuffYu WangShruti Nayak4 more author(s)...
- Description
This study performed a chemical genetic screen to identify PIM1 substrates in prostate cancer cells. They identified 25 previously unknown PIM1 substrates and their phosphorylation sites. Among the identified PIM1 substrates, N-Myc Downstream-Regulated Gene 1 (NDRG1) was shown to be as an inhibitor of metastasis. The study demonstrated that PIM1 phosphorylation of NDRG1 reduced its stability, nuclear...
- Subject
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CancerProteomics
- Access Rights
- Free to All
