Energetics of the Microsporidian Polar Tube Invasion Machinery
- Description
Microsporidia are obligate intracellular parasites that use an unusual invasion organelle called the polar tube (PT), which is ejected from a dormant spore at ultra-fast speeds, to infect host cells. This study used serial block-face scanning electron microscopy (SBF-SEM) to capture 3-dimensional snapshots of Anncaliia algerae spores in different states of the PT ejection process. They proposed a theoretical framework starting with a systematic exploration of possible topological connectivity amongst organelles and assessed the energy requirements of the resulting models by performing PT firing experiments in media of varying viscosity and using the results to rank the proposed hypotheses based on their predicted energy requirement. This dataset contains SBF-SEM and live-cell imaging data.
Access
- Restrictions
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Free to All
- Instructions
- SBF-SEM data is available in EMPIAR and live-cell imaging data of methylcellulose germination experiments have been deposited in Zenodo. The code used in this study, including the analysis of rheometer data, and the calculation of pressure, power and total energy for each hypothesis, is available on GitHub.
- Grant Support
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AHA Postdoctoral Fellowship/American Heart AssociationIrma T. Hirschl Career Scientist Award/Irma T. Hirschl and Monique Weill-Caulier TrustNYU Dean’s Undergraduate Research Fund/NYU College of Arts & ScienceSSP-2018-2737/Searle Scholars ProgramPEW-00033055/Pew Charitable TrustsStanford Bio-X Graduate Fellowship/Stanford UniversityMinistry of Education of the People's Republic of China/Ministry of Education of the People's Republic of ChinaCZ Biohub Investigator Program/Chan Zuckerberg Biohub NetworkSchmidt Innovation Grant/Schmidt FuturesMoore Foundation Research Grant/Gordon and Betty Moore FoundationHHMI Faculty Fellowship/Howard Hughes Medical Institute